Play free essay sample

Mobys â€Å"Play† came out in 1999 and is one of the highest grossing CDs of all time. Moby shows off his incredible talents by writing, engineering, mixing and producing all the songs on this CD. He also plays all the instruments: piano, guitar, flute and drum. From top to bottom, each song is very good with catchy beats and an overall relaxing tone. Even though Moby has refused to make a techno CD, several songs (â€Å"Honey,† â€Å"Find My Baby,† â€Å"Why Does My Heart Feel So Bad† and â€Å"Bodyrock†) fall under the heading of techno. The most popular song here is â€Å"South Side,† featuring Gwen Stefani. They form a good team for a great song. â€Å"Porcelain,† â€Å"Everloving† and â€Å"My Weakness† are beautiful songs that are soothing to the mind and soul. If you want relaxing songs, these are the way to go. â€Å"Run On† continues the variety of the CD, producing a jazzy 1950s sound. We will write a custom essay sample on Play or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page This CD has everything! There is no swearing or negative lyrics on â€Å"Play† so it has broad appeal. This is a must-buy for anyone who likes good music. Go out there and spend the 15 bucks for â€Å"Play.† You wont be disappointed.

Lightning And Static Essays - Electricity, Electrostatics

Lightning And Static Essays - Electricity, Electrostatics Lightning And Static Lightning is a discharge of static electricity in the atmosphere, which causes a bright flash of light. When lighting goes through the air, it heats the air to degrees hotter than the sun's surface about 50,000 degrees fahrenheit. This heating causes the air to expand and contract. This causes the sound called thunder. Lightning is caused by a build up of negative ions in the atmosphere which discharges into the ground causing the negative ions to go with the positive ions in the ground. Static electricity is negatively and positively charged atoms. A static charge on the other hand is a individual atom with a negative or positive charge. There are three types of charges negative positive and neutral. Negative and positive ions attract. But two negatives repel each other, just like two positives will. Neutrally charged ions can attract to both negative and positive ions, because they have and balanced charge of negative and positive ions, this means it has the same amount of negative and positive ions. This is where the following rule is true opposites attract, and likes repel. Static electricity is generated when two different objects are rubbed together and some electrons are stolen from one object and then start to collect on the surface of the other object. The object that loses electrons becomes positively charged, because electrons are negatively charged and there is an absence of electrons and too many protons. Now because opposites attract and like repel the reason hair stands up when you rub something on it can be explained. When something is rubbed in a dry place, the thing takes electrons from your hair, leaving your hair with a positive charge it stands up as far away as possible from the other positively charged hairs. There reason there is little static electricity in areas with a high humidity, like the Virgin Islands, is because the humidity in the air is a conductor of electricity, so it helps trancfer the free electrons to an area that can accept electrons, like the earth so not many free electrons stay on any object that is saturated. Static electricity can be use to do many things, one of these things is keeping down air pollution by putting an electrode in factory smoke stacks so that the dust will collect on the electrode. Lightning and static can be hazardous. lightning is harmful, some people think it does not have any threat from a distance, like when a lighting storm is up to ten miles away. The most lightning strikes take place under the lightning storm where the rain is raining the hardest. Signs to tell if lightning is going to strike in your area are, If your hair stands up you hear static popping or crackling, and if you feel you body tingle due to ions moving through your body the static on portable electronic things increases suddenly, you smell the smell of statically charged air, or you see static sparks sparking in the air around you. Things to do inside to keep from getting hurt while in a lightning storm are, get away from doorways outside, porches, windows, and good conductors of electricity. Avoid phones that are not cordless and if it is a cordless phone avoid it if it is on the cradle. Do not touch things that use electricity unless they are unplugged. Do not stand near or lay on concrete walls. Do not use a metal building for shelter. And don't go into the basement because the electricity can travel through the ground. To not get hurt outside, go inside if you hear thunder, if the sky looks threatening, and stay inside for at least 30 minutes after the la st sound of thunder. If you are caught outside and do not have shelter close to you during a lightning storm, you should crouch to the ground, get onto the balls of your feet, put your hands on your knees, put your head between your knees, make yourself as small as you can, and try to have as little physical contact to the ground as you can. Static electricity has been known about for some time. Some time around the year 600BC the Greek Mathematician

A Complete Guide to 2019 Interview Questions

A Complete Guide to 2019 Interview Questions The hardest part of preparing for an interview is the unknown factor. Although you can make assumptions about what you’ll be asked, you never know for sure until you’re there on the hot seat. Sure, you’ll be asked about your jobs, your skills, and your experience- but there are lots of different ways any given interviewer can go. While it is impossible to predict with 100% accuracy what might be thrown your way, you can do your best to prepare for the different scenarios you might see on interview day. The most asked interview questionLet’s start with THE interview question. The alpha. Without a doubt, the most  asked  interview question in existence is â€Å"Tell me about yourself.† It’s an easy ice-breaker, and it lets the interviewer start to get a sense of what you’re like as a candidate.Here’s how you can answer it:Tell Me About YourselfYour Elevator PitchThis  will most likely be the first question you’re aske d in any job interview, so it’s absolutely imperative you have your response ready to go.Interview questions measuring personalityUnlike the classic â€Å"Tell me about yourself,† questions measuring personality are a lot more uncommon, subtle, and complex. These are generally questions the employer has tailored to measure your personality and how  it would translate to the responsibilities of  their open position.Here are some examples:Ethical Interview QuestionsBehavioral Interview QuestionsHypothetical Interview QuestionsBrainteaser Interview QuestionsSituational Interview QuestionsProblem Solving QuestionsThese questions can be tricky because it’s not often clear what kind of answer the employer wants to hear. A person hiring someone for an accounting position may consider someone with an introverted personality a better fit for the job than someone who parties it up every Saturday, and vice versa.Interview questions you’re not expectingIt’ s less likely that you’ll be asked any of these interview questions, but as you can never be sure of anything in the interview game.  It’s always a good idea to prepare:Interview Questions You’re Not ExpectingRemember, these questions exist, which means they were all asked at one point or another. Don’t be the interviewee  that gets caught like a deer in headlights when faced with a  Ã¢â‚¬Å"gotcha† question.Here are some additional questions that you might not be expecting:â€Å"How would you fire someone?†Ã¢â‚¬Å"What’s your idea of the perfect day?â€Å"â€Å"You’re driving down a dark road, during a wild storm. You drive past a bench†¦.â€Å"The â€Å"biggest challenge† interview questionIf the interviewer does ask you a question like, â€Å"What’s the most difficult part of being a [current job title]?† or â€Å"What was the toughest part of your last job?† here are ways you can frame your struggles  to make yourself look awesome:The Biggest Challenge Interview QuestionsQuestions relating to salaryThe job application process has a number of separate stages: applying, interviewing, job offer, then salary negotiation and acceptance. Most employers stick to this, and let the money talk wait until things are further along. But if they jump the gun and ask you up front for a) your current salary or b) desired salary, what do you do?Interview Questions Relating to SalaryInterview Questions About Your Current SalaryQuestions about salary are important to keep in mind. More and more states are passing legislation about what potential employers can and can’t ask you about your salary history, so make sure you know what your state’s current policies are, before you hand over that information to an interviewer.Illegal interview questionsOut of ignorance or slyness, an interviewer might try to get information out of you that the company is not allowed to fac tor into their hiring decisions. However, you’re not obligated to answer them- and in fact you shouldn’t.  Your battle plan should be to figure out why they’re asking (whether it has direct consequences for the job itself), and to decide whether you should answer.Illegal Interview Questions11 Unethical Interview Questions You Don’t Need to AnswerRed Flag QuestionsSome of the hardest questions you’ll encounter might be illegal, so it’s important to know what the danger zone questions are, and why you don’t need to answer them. And if there’s an out-of-bounds question being asked, don’t panic. Just say you don’t feel comfortable answering it, and deftly steer the topic to something else you’d like to share about yourself.The hardest types of questionsWhen you’re in the hot seat, all questions thrown your way seem tough. Some, though, are harder than others, like the ones below. Remember: your intervi ewer not only wants to get a good sense of what you will answer, but also how you handle the pressure. Stay cool and take a breath, even when you’re struggling to come up with an answer. Study these questions and prep yourself for whatever might come your way.Tough Interview Questions That Are Very CommonThe Hardest Interview Questions of 2016The 10 Toughest Interview QuestionsHow to Answer the Toughest Interview Questions9 Super-Tricky Job Interview Questions and How to Answer ThemWild card questionsAnd finally, here are some additional questions that did not fall into any particular category, but are very important to prep for:â€Å"Can I contact your current employer?â€Å"â€Å"Why have you had so many jobs?â€Å"â€Å"Aren’t you overqualified for this position?â€Å"â€Å"Why Should I Hire You?â€Å"What you should ask themAnd just when you think you’re done prepping for every possible question you can get in an interview, there’s still more work to be done: you need to prep the questions you’re going to ask too. You should always, always have questions for your interviewer.4 Questions to Ask at the End of an InterviewAsk These 10 Interview Questions to Impress Your Hiring ManagerThe Most Important Question You Need to Ask in an InterviewNow that you’re prepared for what you’ll need to say and ask on interview day, don’t forget to dust off your lucky interview outfit and brush up on that body language as well. Good luck, and happy interviewing!

Civil War Essay Example | Topics and Well Written Essays - 500 words - 4

Civil War - Essay Example law was put forward as a measure to placate the South which was upset over the increasing number of free states and anti-slavery sentiments penetrating the government. The law enforced fines on all law personnel who did not arrest a run slave. Prior to this act, the fugitive slave act of 1793 was in action which demanded the return of run-away slaves from the free states. In other words, it was asking the North which termed slavery illegal to abide by the slavery laws of the South. This was in direct contrast with the Northern sentiments. In many states like Massachusetts that had abolished slavery during the eighteenth century, the idea of returning slaves was met with great resistance. The law was not only dangerous for the run-away slaves but also for the free blacks, people who were born and raised in the Free states. The law could easily be misused and these people could then be shipped to the South having been unjustly termed as run-away slaves. The law was not acceptable to the North as it was asking them to become part of enforcing slavery throughout the country. The intention of this law was exacerbate the ensuing tension between the North and the South, however it further increased the divide. The law gave rise to many abolitionist leader like Frederick Douglas who raised their voice actively against the discrimination. The fugitive slave act was soon followed by the Kansas Nebraska act in the 1854 which further increased the divide. The ultimate blow was however dealt during the Kansas killings when proslavery as well as antislavery supporters flooded from the North as well as the South to cast their votes. The ultimate result of all the chaos and bloodshed was the civil war itself which decided once and for all the fate of slavery in the country. The Civil War can undoubtedly be called as one of the most influential events in American history whose effects helped shape the American nation. It is important to study the causes of an event which is of

Career Action Plan PartB Assignment Example | Topics and Well Written Essays - 4000 words

Career Action Plan PartB - Assignment Example A marketability analysis is also included in this particular study for outlining my marketable skills. These identified skills would be compared with wide array of attributes in context of professional industry. There are appropriate analytical techniques incorporated to judge current position of an individual and what others skills are essential so as to secure a strong position in career path. In this study two kinds of attributes are majorly highlighted, one that is already being possessed and other is what is needed to be improved. Ideal situation will be well analyzed by implementing an effective tool known as SWOT analysis. This form of analysis shall encompass both current and ideal situation which is required for designing the best career action plan. Apart from identifying internal strengths and weaknesses and outlining external threats and opportunities, focus will also be on goal setting. In the entire process two forms of goals will be determined like long term and short term goals. At the end of this study, some steps would be discussed for achieving success in career path. Career goals can be explored only when desirable qualities are present within an individual. Career Action Plan is an important tool by which an individual is able to set and develop the steps for fulfilling its goal and objectives in his professional life. He is only responsible to build his own career. But there are certain things involves in career development which requires professional guidance for making decisions and planning of career more effective. It will help the individual to achieve its career goals in an efficient way. A person should start to build his career actions plans from a very early stage. This will help him to develop his employable skills. Various options arise while forming career actions plan. An individual explores each and every option very

The Limits of Amylase Essay Example for Free

The Limits of Amylase Essay Abstract This report explains the purpose of this experiment in a way that conveys information to the reader about Amylase’s ability to withstand acidic or basic pH. To do this, two test tubes were both filled with 5mL of a 5% amylase solution. The first one was filled with an acid, while the other was filled with a base. After dropping liquid Iodine and Benedict’s solution into each one, the tube with a basic pH tested positive for glucose. The acidic solution tested (mostly) negative for glucose, although there were trace amounts at the bottom. Overall, my conclusion is that only acidic solutions inhibit Amylase’s ability to digest starch. Background The goal of the experiment is to test whether or not Amylase can withstand abnormal changes in pH without denaturing itself. Salivary Amylase is an enzyme that acts upon any polysaccharide that enters the mouth (mainly starch). However, it has a few other clinical uses. It is used for the diagnosis of acute pancreatitis, an inflammation of the pancreas, and other medical conditions regarding Amylase levels in the body. Amylase’s first discovery in 1831 led to new experiments about this enzyme. By separating pancreatic Amylase from Trypsin, they were able to realize that it acted upon starch, breaking it down into a simple sugar, or monosaccharide. In the diagram (featured below), the active site of this enzyme contain three major acidic groups. These contain a calcium ion (the large gray sphere) , the chloride ion (the large green sphere), and the chain of five sugar units (in yellow orange). Previously mentioned, this enzyme will act upon starch and break it down into simple sugars. 2 Hypothesis If we expose Amylase to a variety of acidic and basic solutions to test whether it will still digest starch or not, then I think that it will denature both ways, because Amylase is normally intolerable to any pH above 8 or below 6. Materials and Procedures The following will be useful in this experiment: 20mL of a 5% Amylase solution 20mL of a 1% starch solution 8 test tubes At least 5mL of HCl At least 5mL of NaOH Hot plate Beaker full of water (for incubation) Liquid Iodine Solution Benedict’s Solution Follow this procedure: 1. In your first 4 test tubes, add the following solutions accordingly to each tube: -Test tube 1: 5mL of distilled water -Test tube 2: 5mL of the 5% Amylase solution -Test tube 3: 5mL of Amylase solution + 2-3 drops of HCl -Test tube 4: 5mL of Amylase solution + 2-3 drops of NaOH 2. Add 5mL of the starch solution into each tube. 3. Incubate each tube in the beaker of water for 30 minutes. The temperature should be 37 °C (98.6 °F). 4. After incubation, split half of all contents in each tube into 4 new tubes. 5. Insert 2-3 drops of the Iodine solution into the first set of beakers (the original 4). Then, insert 2-3 drops of the Benedicts solution into the second set of test tubes. 6. Record any color changes. 3 Results The first test tube (containing distilled water) was light brown when mixed with iodine, and blue when mixed with Benedict’s solution. The second test tube (5% Amylase solution) was light brown when mixed with iodine. It became orange when Benedict’s was added to the mix. The third test tube turned dark brown when the iodine was added. The other beaker with test tube 3’s contents remained blue when Benedict’s solution was added, although traces of Orange were found. The last test tube appeared Light Brown with the iodine mixed in (3 drops are always used), and appeared Orange when combined with the Benedict’s solution. Discussion Many of the test tubes ended up looking like I expected them to, except the fourth beaker. I had not expected that Amylase, under the influence of a very basic solution, could still act as a catalyst for starch. With a pH of  9 or higher, Amylase would normally denature itself. This could mean one of two things: Amylase is capable of functioning normally under a very high pH, or it could’ve been human error that led to this surprising result. To minimize chances of this happening again, assuming that this was human error only, would be to maximize the pH in the fourth beaker to 14 instead of 12, should a new lab arise, focusing on Amylase’s ability to withstand a high pH without getting denatured. Conclusion Overall, most of my results agreed with my notes and background knowledge about Amylase, apart from the difference in test tube 4. Like I predicted, the Amylase solution (tube 2) tested positive for simple sugars, the acidic solution denatured the enzyme, and the distilled water did absolutely nothing. Sources Dugdale, D. (2013, October 31). Amylase blood. Retrieved from http://www.nlm.nih.gov/medlineplus/ency/article/003464.htm (n.a.) Alpha- amylases. (2006, February 18). Retrieved from http://www.rcsb.org/pdb/education_discussion/molecule_of_the_month/download/Alpha-amylase.pdf (n.a.) Alpha amylase. (2010, January 29). Retrieved from http://science.marshall.edu/murraye/alpha_amylase.htm

Essay examples --

In the given constraints of Cost, Bulkiness and Portability we will design a smart phone based Fundus imaging device. Device application software will solve the more problem like: Automatic Glaucoma Detection and Image Enhancement problem so that its doesn't required trained user for operation. That will be possible by embedding the Optical(image capturing unit), Electronic (Microprocessor), Communication media(Wi-fi,Blue-tooth,Web etc) and Data base in a single device (Figure 2.1). This section will capture magnified fundus image in CMOS Camera. To make its possible by interfacing direct ophthalmoscope with CMOS Camera.Ophthalmoscope is an instrument that is use for examining the interior structures of the eye, especially the retina, consisting essentially of a mirror that reflects light into the eye and a central hole through which the eye is examined. Here it will interface device and human eye. We will used smart phone high resolution ($>$ 5MP) Camera for the same. Use a mobile phone holder (Rapid Prototype Model) to mount Mobile phone camera on ophthalmoscope. section{Image Accusation and Data management} Optical image of the fundus is converted into digital form by Mobile phone inbuilt Camera. Initially capture image/video is saved in local on board memory or SD Card. This section handles the data organization and management work such that we can easily find out any patient data.To organized data it will do flowing task: egin{enumerate} item{Create Folder whose Name is same Patient ID} item{Save Image/Video in that folder} item{Right and Eye Information is used to do file naming .} end{enumerate} section{Image Processing} This section will perform all the image processing task, that is widely separated into two ... ...paration of around 10 mm. The ophthalmoscope used provides an easy entry into the eye, together with a wider field of view to better observe eye conditions. paragraph{} The unit captures the fundus image in a JPEG format, which gets stored in the phone. The image is then processed for detection of its optical disk and cup, after which the respective areas are calculated to compute the CDR for setting up the threshold for the affected eye. The image processing operations as discussed above are inbuilt in the Android Application. paragraph{} The setup for capturing the fundus image of a person's eye using the developed system is shown in figure 2.2. The system is held very close to the person's eye to be detected for Glaucoma. The real time application on the phone displays the image. Also, it prints out the result in a format which can easily be interpreted.

Department of biology Essay

INTRODUCTION: Every cells of living organism contains genetic materials known as deoxyribonucleic acid, DNA. It can be isolated from tissue sample of living things by separating it from other cellular component in a manner that still preserves its structures. The structure of DNA is double-stranded helices that made up from the monomer of nucleotides. Each of the nucleotides composed of three parts that are the phosphate group, deoxyribose sugar backbone and also nitrogenous bases (Adenine, Guanine, Thymine and Cytosine). This nitrogenous base are arranged in sequence and holds the information and coding for controlling the physical trait that we all have as well as the regulation of our body. DNA extraction is simply a process that results in separation of DNA from the cells or viruses that are hosting it. Through the meaning is simple but the process is not. When we go into the peculiar details of DNA extraction, we realize that it’s more of an initial stage in other intensive DNA testing processes. DNA test could be performed for any reason; however for any DNA testing to happen the first stage normally is the isolation and extraction of DNA molecules from the cells that they reside in. DNA extraction follows a series of step, stripping all proteins from the DNA and the extraction protocols have to make sure that the DNA thus obtained via isolation and extraction is of high or acceptable quality. DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical methods. Currently it is a routine procedure in molecular biology or forensic analysis. Primary structure consists of linear sequence of nucleotides that are linked together by phospodiester bonds. It is the linear sequence of nucleotides that make up the primary structure of DNA. Specific techniques must be chosen for isolation of DNA from some samples such as samples from microorganisms with thick cellular wall, for example yeast. Biological DNA represents the information which directs the functions of a living thing. A. Yeast DNA Extraction Materials : 1 packet of dry yeast, Sodium chloride, Meat tenderizer, Ice cold 95% ethanol, Sunlight detergent, distilled water, blender, graduate cylinders ( 10mL, 100mL and 500mL ), Beaker ( 250mL, 100mL ), glass stirring rod and wooden sticks, 15mL test tube, test tube rack, 1 ,l pipette and blue tips. Method: 1. 1 packet of dry yeast was mixed with 40ml of 50Â °C tap water. The yeast was stir to dissolve and the mixture was leave and covered for 20 minutes. 2. Salt/detergent solution was prepared by adding 40 ml detergent and 40g NaCl to 360ml distilled water. The solution was mixed till dissolved. 3. 5% meat tenderizer solutions were prepared by adding 5g of meat tenderizer to 80 ml of distilled water. The solution was top up to 100 ml with distilled water. *salt / detergent solution and meat tenderizer solution is prepared once for Part A, B and C. *alternatively, 5% meat tenderizer solution may be substitute with 100 ml of fresh papaya juice or pineapple juice. 4. 40 ml of yeast mixture and 40 ml of salt/detergent solutions was placed in a blender and was blended at high speed for 2 minutes. 5. The solution was pour into the beaker and 15ml of meat tenderizer solution was added. The solution was stir to mix. 6. The mixture was leave at room temperature for 5 minutes. 7. A cheese cloth was place over a filter funnel. The mixture was pour over the filter funnel and the clear supernatant was collected. 8. 3 ml of clear solutions was transfer into a 15ml tube. 9. The test tube was tilted to a 45 degree position. 3 ml of 95% ice cold ethanol was gently added to the side of the tube. 10. The test tube was leave undisturbed for 3-5 minutes. A layer will be formed in the tube. 11. DNA precipitate was formed at the interphase layer. A wooden stick was used to swirl the DNA out Result : Table A(i) : Yeast DNA Extraction B. Onion DNA Extraction Materials : Fresh onion, salt detergent solution, meat tenderizer solution, ice cold 95% ethanol, distilled water, blender, graduated cylinders (10 ml and 100 ml), glass stirring rod and wooden sticks, 15 ml test tube, test tube rack, 1 ml pipette and blue tips. Method : 1. The prepared salt/detergent solutions and meat tenderizer solution from part A was gathered. 2. 3 medium sized onions were cut into an inch cube and were placed in a blender. 3. 100 ml of salt/detergent solution was added in a blender. 4. The solution was blended at high speed for 2 minutes. 5. A cheese cloth was placed over filter funnel. The mixtures were poured over the filter funnel and the clear supernatant was collected. 6. The clear solution was transfer into a beaker and 30 ml of meat tenderizer solution was added. 7. The mixtures were leave at room temperature for 5 minutes. 8. 3 ml of clear solutions was transfer into a 15 ml tube. 9. The test tube was tilted to a 45 degree position. 3 ml of 95% ice cold ethanol was gently added to the side of the tube. 10. The test tube was leave undisturbed for 3-5 minutes. A layer will be formed in the tube. 11. DNA precipitate was formed at the interphase layer. A wooden stick was used to swirl the DNA out. Result : Table B(i) : Onion DNA Extraction C. Apple and Orange DNA extraction Materials : Fresh apple, fresh orange, salt detergent solution, meat tenderizer solution, ice cold 95% ethanol, distilled water, blender, graduated cylinder ( 10 ml and 100 ml ), glass stirring rod and wooden sticks, 15 ml test tube, test tube rack, 1 ml pipette and blue tips. Methods : 1. The prepared salt/detergent solutions and meat tenderizer solution from part A was gathered. 2. An apple / orange were cut into an inch cube and were placed in blender. 3. 100 ml salt/detergent solutions were added in a blender. 4. The solution was blended at high speed for 2 minutes. 5. A cheese cloth was placed over filter funnel. The mixtures were poured over the filter funnel and the clear supernatant was collected. 6. The clear solution was transfer into a beaker and 30 ml of meat tenderizer solution was added. 7. The mixtures were leave at room temperature for 5 minutes. 8. 3 ml of clear solutions was transfer into a 15 ml tube. 9. The test tube was tilted to a 45 degree position. 3 ml of 95% ice cold ethanol was gently added to the side of the tube. 10. The test tube was leave undisturbed for 3-5 minutes. A layer will be formed in the tube. 11. DNA precipitate was formed at the interphase layer. A wooden stick was used to swirl the DNA out. Result : Table C(i) : Orange DNA Extraction Table C (ii) : Apple DNA Extraction RESULT ALL OF EXPERIMENTS: Sample Extraction Amount Apple Success Large Onion Success Small Orange Success Small Yeast Success Small DISCUSSION: Based on our experiment discussion, we obtained that the difference amount of DNA extraction from yeast, onion, apple and orange is different. The amount of apple DNA extraction is larger than onion, orange and apple DNA extraction. Each step of the process will help in a certain way to extract DNA, until we are finally successful in the end. In the yeast, extraction, the ethanol will be able to separate the DNA and it will float between the less dense ethanol and the denser homogenizing mixture. In the onion DNA extraction, the chloroform and homogenizing medium will help break down the cell membranes and the ethanol like in the yeast DNA extraction. It will cause the DNA to separate and be suspended in the interface between the two solutions. It will be same to apple and orange DNA extraction. The precaution step on this experiments we tilt 45Â º to add the ethanol. This is because it will form a layer on top of the sample because the ethanol is less than water. So, it does will be on the top of layer. We also used 50ï‚ ° of hot water in yeast solution. Its will formed the best of result because hot water is the optimum temperature for yeast. We also obtained about perceptions step why only clear solution produced in these experiments. Its means the extraction was failed. CONCLUSION: REFERENCES: http://www.whatisdna.net/dna-extraction.html http://classic.sidwell.edu/us/science/vlb5/Labs/DNA_Extraction_Lab/dna_extraction_lab.html#predictions http://sciencehk.weebly.com/lab-reports.html ANSWER AND QUESTION: Answer all the questions. 1. Describe the functions of following: a. Applying blender to sample and salt/detergent solutions Strain the DNA mixture. b. Salt Salty water helps the DNA precipitate (solidify and appear) when alcohol is added. c. Detergent Detergents are used to break down cell walls and nuclear membranes to release the DNA. They work by chemically poking holes in the cell membranes or walls. Once holes are poked in the membranes, the membranes can be further distrupted mechanically, as with a blander. After that, it’s easier to get the contents of the cell out, including the DNA, d. Meat tenderizer solutions Meat tenderizer acts as an enzyme. The DNA in the nucleus of the cell is moulded, folded, and protected by proteins. The meat tenderizer cuts the proteins away from the DNA. e. 95% ice cold ethanol Having ice cold ethanol only increases the rate of precipitation of DNA and helps increase yield of DNA. It can either use room temp or ice cold ethanol for DNA precipitation. Think about precipitation of a super concentrated solution as you decrease the temperature. As the temperature decreases, the amount of precipitation increases. Overall temperature affects solubility. As temperature decreases the substance becomes more insoluble (in general. this does not apply to every molecule). So, ice cold EtOH allows for more DNA to interact together and allow for a more rapid and efficient precipitation of DNA. 2. Compare the reliability amount of DNA obtained from all the samples. The reliability amount of DNA obtained from apple is much larger compared to Reliability amount of DNA from onion, orange and apple. 3. Write out the principle involved in DNA extraction. – Break open cells by mashing the fruit. – Dissolve organelles and cell membranes with detergent. – Separate DNA from proteins with salt – Filter out the clumps with a coffee filter – DNA precipitates in cold alcohol and is spooled out.

Different Aspects of Women

In Bram Stoker’s novel, Dracula, Stoker portrays many different aspects of women’s roles in the nineteenth century. Women had a strictly defined role within the era; there was no thought of equality, no thought that women could liberate themselves sexually. Stoker uses women in this novel to critique against women’s liberation. Stoker’s portrayal of women makes the novel seem like a fantasy. Women are primarily objects of delicate beauty who occasionally need to be rescued from danger. In the novel Mina Murray is the embodiment of Victorian virtue in which she is loyal, earnest, innocent, and dependent of her husband.Stoker creates another character, Lucy Westenra who is completely opposite of Mina. Lucy is embodies the desire of women who want to liberate themselves. Only Mina shows any considerable strength or resourcefulness. Lucy is primarily two-dimensional victim, picture of perfection who is easy for Dracula to prey upon. Mina is not most noteworthy for her physical beauty like Lucy. Mina’s sexuality remains mysterious throughout the whole novel of Dracula. Even though she gets married, she never gives voice to anything resembling a sexual desire or impulse, which enables her to retain her purity.Stoker creates suspense about whether Mina will be lost like Lucy. Throughout the novel, Mina portrays what women are expected to do in the society. In the beginning of the novel, Lucy is much like Mina. She is a paragon of virtue and innocence, qualities that brings three suitors to her. Lucy differs from Mina in one aspect, she shows her sexuality. Lucy’s physical appearance captivates each of her suitors, and she displays comfort or playfulness about her desirability that Mina never feels.Stoker amplifies this faint whisper of Lucy’s insatiability to a monstrous volume when he describes the undead Lucy as a wonton creature of ravenous sexual appetite. With Stoker’s concern with female chastity and virtue , it is hard to imagine him granting his female characters the degree of sexual freedom. Lucy’s wounds suggest a virgin’s first sexual encounter. She escapes into the night and is penetrated in a way that makes her bleed. â€Å"As she looked, her eyes blazed with unholy light, and the face became wreathed with a voluptuous smile†¦She still advanced, however, and with a anguorous, voluptuous grace, said: ‘Come to me, Arthur. Leave these others and come to me. My arms are hungry for you. Come, and we can rest together. Come, my husband, come! ’ There was something diabolically sweet in her tones— something of the tingling of glass when struck— which rang through the brains even of us who heard the words addressed to another† ( Stoker 227). This threat becomes reality as Lucy, now a blood and sex starved vampire, does her best to lure her fiance into eternal damnation.Lucy is the first to fall under Dracula’s spell because she displays comfort or playfulness about her desirability that Mina does not show. Stoker also describes her as the â€Å"loose† woman, she isn’t conservative as Mina and she loves all the attention from her suitors. Lucy also falls under Dracula’s spell because Dracula has this charm that attracts Lucy making her have strong desire for Dracula. Lucy shows that she has sex appeal and that she has desirability for men; and it made her an easy target for Dracula, knowing she will give in when he approaches her.

Free Essays on The House Of Seven Gables

â€Å"The sympathy or magnetism among human beings is more subtle and universal than we think; it exists, indeed, among different classes of organized life, and vibrates from one to another. Loosely based on the events of Hawthorne’s own life, The House of the Seven Gables attempts to show the suffering of descendants forced to repent for the sins of their â€Å"father†, while they are unknowingly renewing the curse by nurturing the ancestral greed that has passed through the generations. Thus the various themes of the novel reflect the central idea of continued sin through the greed and guilt of a declining family. Each generation struggles to escape the sins of the past, only to be thrust forcefully back to face the offenses of their forefathers. The House of the Seven Gables is a tale of loneliness and greed caused by the sin of preceding generations. The opening of the novel is set in puritan times during the Salem witch hunts. The villainous Colonel Pyncheon wrongly accused the innocent Matthew Maule of witchcraft so that the Maule land would fall into the Pyncheon family’s hands. Upon his death, Maule â€Å"addressed [Colonel Pyncheon] from the scaffold, and uttered a prophecy...God will give him blood to drink". The physical wrongdoing of Colonel Pyncheon against Matthew Maule was avenged at the former’s death, with the curse being fulfilled. However, the essence of the crime lived on through the generations. By chapter two, the focus of the novel has shifted to the modern generations of the Pyncheon family. The family has severely declined since the Colonel’s time, yet the curse of greed is as strong as ever. The remains of the family consist of a decrepit spinster named Hepzibah, now the caretaker of the house of the seven gables; her insane brother Clifford, who was just recently released from prison; their devilish cousin Judge Jeffrey, a man fixated upon his own greed; and their distant... Free Essays on The House Of Seven Gables Free Essays on The House Of Seven Gables â€Å"The sympathy or magnetism among human beings is more subtle and universal than we think; it exists, indeed, among different classes of organized life, and vibrates from one to another. Loosely based on the events of Hawthorne’s own life, The House of the Seven Gables attempts to show the suffering of descendants forced to repent for the sins of their â€Å"father†, while they are unknowingly renewing the curse by nurturing the ancestral greed that has passed through the generations. Thus the various themes of the novel reflect the central idea of continued sin through the greed and guilt of a declining family. Each generation struggles to escape the sins of the past, only to be thrust forcefully back to face the offenses of their forefathers. The House of the Seven Gables is a tale of loneliness and greed caused by the sin of preceding generations. The opening of the novel is set in puritan times during the Salem witch hunts. The villainous Colonel Pyncheon wrongly accused the innocent Matthew Maule of witchcraft so that the Maule land would fall into the Pyncheon family’s hands. Upon his death, Maule â€Å"addressed [Colonel Pyncheon] from the scaffold, and uttered a prophecy...God will give him blood to drink". The physical wrongdoing of Colonel Pyncheon against Matthew Maule was avenged at the former’s death, with the curse being fulfilled. However, the essence of the crime lived on through the generations. By chapter two, the focus of the novel has shifted to the modern generations of the Pyncheon family. The family has severely declined since the Colonel’s time, yet the curse of greed is as strong as ever. The remains of the family consist of a decrepit spinster named Hepzibah, now the caretaker of the house of the seven gables; her insane brother Clifford, who was just recently released from prison; their devilish cousin Judge Jeffrey, a man fixated upon his own greed; and their distant...

Content and Function Words in English

Content and Function Words in English Each word in English belongs to one of the eight parts of speech. Each word is also either a content word  or a function word. Lets think about what these two types mean: Content Words vs. Function Words Content information, meaningFunction necessary words for grammar In other words, content words give us the most important information while function words are used to stitch those words together. Content Word Types Content words are usually nouns, verbs, adjectives, and adverbs. A noun tells us which object, a verb tells us about the action happening, or the state. Adjectives give us details about objects and people and adverbs tell us how, when or where something is done. Nouns, verbs, adjectives and adverbs give us important information required for understanding. Noun person, place or thingVerb action, stateAdjective describes an object, person, place or thingAdverb tells us how, where or when something happens Examples: Nouns Verbs house enjoy computer purchase student visit lake understand Peter believe science look forward to Adjectives Adverbs heavy slowly difficult carefully careful sometimes expensive thoughtfully soft often fast suddenly Other Content Words While nouns, verbs, adjectives and adverbs are the most important content words, there are a few other words that are also key to understanding. These include negatives like no, not and never; demonstrative pronouns including this, that, these and those;  and question words like what, where, when, how and why. Function Word Types Function words help us connect important information. Function words are important for understanding, but they add little meaning beyond defining the relationship between two words. Function words include auxiliary verbs, prepositions, articles, conjunctions, and pronouns. Auxiliary verbs are used to establish the tense, prepositions show relationships in time and space, articles show us something that is specific or one of many, and pronouns refer to other nouns. Auxiliary verbs do, be, have (help with conjugation of tense)Prepositions show relationships in time and spaceArticles used to indicate specific or non-specific nounsConjunctions words that connectPronouns refer to other nouns Examples: Auxiliary Verbs Prepositions do in has at will though is over has been between did under    Articles Conjunctions Pronouns a and I an but you the for him so us since ours as she Knowing the difference between content and functions words is important because content words are stressed in conversation in English. Function words are non-stressed. In other words, function words are not emphasized in speech, while content words are highlighted. Knowing the difference between content and function words can help you in understanding, and, most importantly, in pronunciation skills. Exercise Decide which words are function and content words in the following sentences. Mary has lived in England for ten years.Hes going to fly to Chicago next week.I dont understand this chapter of the book.The children will be swimming in the ocean this time next week.John had eaten lunch before his colleague arrived.The best time to study is early in the morning or late in the evening.The trees along the river are beginning to blossom.Our friends called us yesterday and asked if wed like to visit them next month.Youll be happy to know that shes decided to take the position.I wont give away your secret. Check your answers below: Exercise Answers Content words are in bold. Mary has lived in England for ten years.Hes going to fly to Chicago next week.I dont understand this chapter of the book.The children will be swimming in the ocean at five oclock.John had eaten lunch before his colleague arrived.The best time to study is early in the morning or late in the evening.The trees along the river are beginning to blossom.Our friends called us yesterday and asked if wed like to visit them next month.Youll be happy to know that shes decided to take the position.I wont give away your secret.

Social Meaning of Vampires Essay Example | Topics and Well Written Essays - 750 words

Social Meaning of Vampires - Essay Example Eighteenth century can be bestowed with the credit for promoting the vampire phenomena after tons of vampire superstitions flew into Western Europe. However, it was Bram stoker’s vampire novel by the name of Dracula that greatly popularized the modern vampire superstition. When questioned about the image of Vampires or Dracula to be more precise, plentiful of people will be of the opinion that Vampires are nothing but tall, dark and handsome men dressed in a long white or black cape and have blood flowing out of their mouths like a river. Others think that Vampires are young, gorgeous, sensual and young women who seduce people, men mostly, in their beds at night and then succeed to suck their blood. This wide realm of opinions proves the very noticeable fact that the social meaning of vampires and the cultural meaning of vampires significantly fluctuate from one area to the other, one region to the other and one continent to the other. To begin with, in ancient Babylonia, people faithfully believed that there existed a female vampire popularly known as Lilu and Lilu was seen feeding and nourishing the pregnant women and at times the newborn babies in that region. On the other hand, in Slavic regions, the residents of that area believed that vampires took birth due to a collection of reasons and some of those reasons were offensive burial rituals and practices and dying an â€Å"unnatural death. (Vampires: The Origin of the Myth by Adrian Nicholas McGrath) In this increasingly modernizing world, as science and technology advances with every passing second, several cultural interpretations exist revolving around vampires. Various cultures and societies place unique concentration and even more distinctive interpretation to these deadly creatures. However, there exists an overlapping section where one can find some of